pnpp phosphatase assay protocol

ELISA technical guide and protocols

Two enzymes are commonly used in ELISA applications Alkaline Phosphatase (AP) is a large enzyme used in a minority of assays Its size (140 KDa) makes it difficult to conjugate more than one or two molecules of the enzyme to each molecule of an antibody or avidin and this limits the amount of signal that can be generated AP is also prone to

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Alkaline Phosphatase Colorimetric Assay Kit

Alkaline Phosphatase Colorimetric Assay Kit (Catalog # GTX85593 500 Reactions Store kit at –20℃) I Reagent Reconstitution and General Consideration: pNPP Solution: Dissolve 2 tablets pNPP into 5 4 ml Assay Buffer to make 5 mM work solution Two tablets are sufficient for 100 assays NEVER TOUCH THE TABLETS WITH BARE HANDS

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SensoLyte™ pNPP Alkaline Phosphatase ELISA Assay Kit

SensoLyte™ pNPP Alkaline Phosphatase ELISA Assay Kit *Colorimetric* Catalog Number: 71232-R: Components: 1 pNPP chromogenic substrate (absorption maximum = 405 nm upon dephosphorylation) 2 Assay buffer 3 ELISA wash buffer 4 Stop solution 5 Alkaline phosphate-conjugated secondary antibody (goat anti-rabbit IgG) 6 An optimized ELISA assay protocol

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SensoLyte pNPP Alkaline Phosphatase Assay Kit AS

This SensoLyte kit can be used to detect alkaline phosphatase activity in biological samples and alkaline phosphatase-based ELISA The kit contains: pNPP chromogenic liquid substrate (absorption maximum = 405 nm upon dephosphorylation) Assay buffer Stop solution Alkaline phosphatase standard mix and read assay protocol that is compatible with high throughput screening Kit size: 500 assays

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pNPP Microwell Alkaline Phosphatase Stablized Substrate

pNPP Alkaline Phosphatase Microwell Substrate (p-nitrophenylphosphate) is a soluble substrate used with the enzyme alkaline phosphatase designed for various qualitative or quantitative immunoassays but not recommended for membrane or immunohistochemical applications where a precipitating reaction product is required The substrate is supplied as a one component

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SensoLyte pNPP Alkaline Phosphatase Assay Kit

The kit contains: pNPP chromogenic liquid substrate (absorption maximum = 405 nm upon dephosphorylation) Assay buffer Stop solution Alkaline phosphatase standard "mix and read" assay protocol that is compatible with high throughput screening

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New England Biolabs (UK) Ltd

The PNPP phosphatase activity is measured using a continuous or single-point spectrophotometric assay based on the ability of phosphatases to catalyze the hydrolysis of PNPP to p-nitrophenol a chromogenic product with absorbance at 405 nm (1) The assay can be used for the quick analysis of the protein phosphatase activity under any non-standard

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Use of p

A simple method of assaying soil phosphatase activity is described It involves colorimetric estimation of the p-nitrophenol released by phosphatase activity when soil is incubated with buffered (pH 65) sodium p-nitrophenyl phosphate solution and toluene at 37 C for 1 hr The method is rapid and precise and it has significant advantages over methods previously proposed for assay

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Lambda Protein Phosphatase (Lambda PP)

Lambda Protein Phosphatase (Lambda PP) is a Mn 2+-dependent protein phosphatase with activity towards phosphorylated serine threonine and tyrosine residues It is the 221 amino-acid product of the ORF221 open reading frame on bacteriophage lambda (1 2)

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PP2A Immunoprecipitation Phosphatase Assay Kit

PP2A Immunoprecipitation Phosphatase Assay Kit can be used to detect purified PP2A or PP2A from a cellular lysate It utilizes the Malachite Green contains components that have been matched to optimize sensitivity range of detection - Find MSDS or SDS a COA data sheets and more information

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A comparative study of alkaline phosphatase activity in

Alkaline phosphatase activity assay Alkaline phosphatase is evident in the outer cortical cells or the external cell walls of these species as was demonstrated by a histochemical stain (Hernfindez et al 1994a) Surface alkaline phosphatase was assayed colorimetrically in three replicate samples by the method of Reichardt et al (1967) which uses p-nitrophenyl phosphate (pNPP)

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p

p-Nitrophenyl Phosphate (PNPP) is a non-proteinaceous non-specific substrate used to assay protein alkaline and acid phosphatases The PNPP phosphatase activity is measured using a continuous or single-point spectrophotometric assay based on the ability of phosphatases to catalyze the hydrolysis of PNPP to p -nitrophenol a chromogenic product with absorbance at

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Use of p

A simple method of assaying soil phosphatase activity is described It involves colorimetric estimation of the p-nitrophenol released by phosphatase activity when soil is incubated with buffered (pH 65) sodium p-nitrophenyl phosphate solution and toluene at 37 C for 1 hr The method is rapid and precise and it has significant advantages over methods previously proposed for assay

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New England Biolabs (UK) Ltd

The PNPP phosphatase activity is measured using a continuous or single-point spectrophotometric assay based on the ability of phosphatases to catalyze the hydrolysis of PNPP to p-nitrophenol a chromogenic product with absorbance at 405 nm (1) The assay can be used for the quick analysis of the protein phosphatase activity under any non-standard

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SensoLyte pNPP Alkaline Phosphatase Assay Kit

Protocol B Detecting alkaline phosphatase activity in ELISA 1 Prepare working solutions Note: For ELISA plate preparation please refer to Appendix II 1 1 1X assay buffer: Dilute 10X assay buffer (Component B) to 1X assay buffer with deionized water 1 2 pNPP alkaline phosphatase substrate working solution: Add 5 mL of pNPP substrate

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SensoLyte pNPP Alkaline Phosphatase ELISA Assay Kit

The protocol can readily be modified to run assays in a 384-well format _____ Protocol 1 Prepare working solutions Note 1: Prepare an ELISA assay plate according to standard ELISA procedures (see Appendix) Alkaline phosphatase-conjugated goat anti-rabbit IgG (Component E) is provided in the kit

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Colorimetric Analysis of Alkaline Phosphatase Activity in

This colorimetric assay is based on the conversion of para-nitrophenyl phosphate (pNPP) to para-nitrophenol resulting in a colored product at 405 nm Compared to other conventional ALP assay such as agarose gel electrophoresis 19 wheat germ agglutinin (WGA) precipitation and WGA-HPLC 20 this assay is highly specific sensitive easy to reproduce and most

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Alkaline Phosphatase Activity Colorimetric Assay Kit

Alkaline Phosphatase Assay Kit is a highly sensitive simple direct and HTS-ready colorimetric assay designed to measure ALP activity in serum and biological samples It contains 10 substrate tablets providing convenience for multiple usages The kit uses p-nitrophenyl phosphate (p NPP) as a phosphatase substrate which turns yellow (λ max = 405 nm) when

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A comparative study of alkaline phosphatase activity in

Alkaline phosphatase activity assay Alkaline phosphatase is evident in the outer cortical cells or the external cell walls of these species as was demonstrated by a histochemical stain (Hernfindez et al 1994a) Surface alkaline phosphatase was assayed colorimetrically in three replicate samples by the method of Reichardt et al (1967) which uses p-nitrophenyl phosphate (pNPP)

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Alkaline Phosphatase Colorimetric Assay Kit

Alkaline Phosphatase Colorimetric Assay Kit (Catalog # GTX85593 500 Reactions Store kit at –20℃) I Reagent Reconstitution and General Consideration: pNPP Solution: Dissolve 2 tablets pNPP into 5 4 ml Assay Buffer to make 5 mM work solution Two tablets are sufficient for 100 assays NEVER TOUCH THE TABLETS WITH BARE HANDS

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pNPP Stop Solution

pNPP substrates react with alkaline phosphatase yielding a soluble yellow reaction product Stop Solution for AP Substrates stabilizes the reaction for one hour and does not change the color or the absorbance of the yellow chromogen The absorbance is read at 405 nm to 420 nm

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SensoLyte pNPP Alkaline Phosphatase Assay Kit

1 1 1X assay buffer: Dilute 10X assay buffer (Component B) to 1X assay buffer with deionized water 1 2 pNPP alkaline phosphatase substrate working solution: Add 5 mL of pNPP substrate solution (Component A) to 5 mL of 1X assay buffer This amount of

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Acid Phosphatase Activity Colorimetric Assay Kit

Phosphatase Assay Kit is a high sensitivity simple direct and HTS-ready colorimetric assay designed to measure AP activity in serum and other samples It is suitable for research and drug discovery The kit uses p-nitrophenyl phosphate (pNPP) as a phosphatase substrate which turns yellow (λ max = 405 nm) when dephosphorylated by AP The kit can detect as low as 20 U acid phosphatase

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EnzChek Phosphatase Assay Kit

MUP or 4-nitrophenyl phosphate (PNPP) Each kit contains suf-ficient substrate for ~1000 assays in a fluorescence mi-croplate reader using a reaction volume of 100 L per assay The kit also contains reaction buffer 6 8-difluoro-7-hydroxy-4-methyl-coumarin for use as a reference standard and potato acid phos-phatase for use as a positive control (Figure 3) Fluorescence

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